Brochure Download Form

File Name: [ MB]
   
Name :*
   
Email ID :*
   
Mobile :*
   
 
 
×

Enquiry / Feedback Form

 
   
Name :*
   
Institute :*
   
Address :*
   
Email :*
   
Mobile :*
   
Description :
   
Type :*
   
 
 
×

Molecular DNA Cloning Services

The molecular cloning team of SciGenom offers customized plasmid cloning services, and provides the finished ready-to-use DNA constructs. Currently, our service cater to the following molecular cloning needs

Regular PCR-Insert Cloning:

PCR Amplification of specific DNA fragments from genomic DNA / cDNA libraries and cloning into a standard vector or into the preferred vector of customer.

DNA sub-cloning:

Restriction digestion of DNA inserts and sub-clone into specific vectors of interest, viz. for in vivo protein expression, for construction of hybrid genes, etc

Site-directed Mutagenesis:

Design and synthesis of mutagenic primers, mutagenesis reaction setup and Sanger sequencing to confirm desired DNA mutations.

Multi-DNA fragments Assembly and Cloning:

Design of modular DNA fragments and construction of combinatorial gene variants using DNA assembly techniques.

Cloning in E.coli expression vectors:

Customers can choose from the varied promoter types and fusion tags; and get their target genes cloned into these engineered vectors (refer the schematic representation image). These clones are ideal for protein over-expression studies in E.coli.
ecoli

Cloning of CRISPR-gRNA constructs and HR donor vectors:

Design of gRNAs, synthesis and cloning into any preferred vector of the customer. Design and clone the homology arms into donor plasmid for the knock-out, knock-in, and other CRISPR-Cas9 gene-editing modifications.

Regular PCR-Insert Cloning

   
Identity of the insert sequence to be cloned:*
   
Sequence of the insert to be cloned:*
   
Whether gene synthesis of insert is desired?:* Yes No
   
Whether source insert DNA is provided by the customer?:* Yes No
   
Whether vector DNA is provided by the customer?:* Yes No
   
Select Vector:*
   
Vector Name :*
   
Vector Size :*
   
Vector Sequence :
   
Cloning site to be utilized :* RE1 RE2
   
Additional info to be included:
 
Note: Source insert and destination vector to be provided by customer
 

Contact Details

   
Contact Name:*
   
Contact Email:*
   
Contact Phone:*
   
Institute:*
   
 
 
×

DNA sub-cloning

   
Identity of the insert sequence to be cloned:*
   
Sequence of the insert to be cloned:*
   
Restriction enzyme site to be used for cloning:* RE1:  RE2:
   
Identity of the source vector:*
   
Size of the source vector: *
   
Antibiotic resistance: *
   
Copy Number:
   
Sequencing primer info: Forward:  

Reverse:
   
Identity of the destination vector: *
   
Sequence of the destination vector:
   
Cloning sites to use in destination vector: * RE1:  RE2:
   
Insert orientation in destination vector:
   
Antibiotic resistance: *
   
Copy Number:
   
Sequencing primer info: Forward:  

Reverse:
   
Sequence verification to be done for:*
   
Note: Source vector and destination vector to be provided by customer
 

Contact Details

   
Contact Name:*
   
Contact Email:*
   
Contact Phone:*
   
Institute:*
   
 
 
×

Site-directed mutagenesis

   
Identity of the original insert:*
   
Size of the original insert:*
   
Complete sequence of the original insert:*
   
Total size of the original plasmid: *
   
Complete sequence of the original plasmid:
   
Nature of the desired mutation:*
   
Provide text file with description of the mutations:
(File types : csv| xlsx | xls | docx | doc | rtf | txt)
(File size : 2MB Max.)
   
Sequence verification to be done for:*
   
Whether subcloning of mutant insert into new destination vector is desired?:* Yes No
   
Identity of the destination vector: *
   
Sequence of the destination vector:
   
Cloning sites to use in destination vector: * RE1:  RE2:
   
Insert orientation in destination vector:
   
Antibiotic resistance: *
   
Copy Number:
   
Sequencing primer info: Forward:  

Reverse:
   
Sequence verification to be done for:*
   
Note: Original Insert DNA to be provided by customer
 

Contact Details

   
Contact Name:*
   
Contact Email:*
   
Contact Phone:*
   
Institute:*
   
 
 
×

Multi-DNA fragments Assembly and Cloning

   
Identity of sequence for DNA assembly: *
   
Number of fragments for DNA assembly: *
   
Sequence for assembled DNA construct: *
   
Sequences of individual DNA fragments
Fragment-1*
   
Fragment-2*
   
Fragment-3*
   
Fragment-4*
   
Whether gene-synthesis of insert is required:* Yes No
   
Identity of the destination vector: *
   
Sequence of the destination vector:
   
Cloning sites to use in destination vector: * RE1:  RE2:
   
Insert orientation in destination vector:
   
Antibiotic resistance: *
   
Copy Number:
   
Sequencing primer info: Forward:  

Reverse:
   
 

Contact Details

   
Contact Name:*
   
Contact Email:*
   
Contact Phone:*
   
Institute:*
   
 
 
×

Cloning in E.coli Expression Vectors

   
Identity of protein sequence:*
   
DNA encoding sequence of the protein:*
   
Whether gene-synthesis of insert is required:* Yes No
   
Whether source insert DNA is provided by customer:* Yes No
   
Preferred choice of the expression promoter:*
   
Preferred choice of the fusion tag:*
   
Additional information to be included:
   
 

Contact Details

   
Contact Name:*
   
Contact Email:*
   
Contact Phone:*
   
Institute:*
   
 
 
×

Cloning of CRISPR-gRNA constructs and HR donor vectors

   
Attach file with details of the project:*
(File types : csv| xlsx | xls | docx | doc | rtf | txt)
(File size : 2MB Max.)
   
 

Contact Details

   
Contact Name:*
   
Contact Email:*
   
Contact Phone:*
   
Institute:*
   
 
×

Enquiry / Feedback Form

 
   
Name :*
   
Institute :*
   
Address :*
   
Email :*
   
Mobile :*
   
Description :
   
Type :*
   
 
 
×