DNA Sequencing: decoding life

SciGenom has a fully equipped state-of-the-art DNA sequencing laboratory. Targeted at Research Institutions, laboratories, Pharma & Biotech companies and Investigators our services are includes a variety of packages that are tailored to our customer needs.

We provide the following services:

dna sequencing

Next generation sequencing machines available in our lab

  • Roche 454 - High through-put and the unique combination of long sequencing reads and exceptional accuracy make the system well suited for the sequencing of genomes that contain repetitive or unknown regions.
  • MiSeq – Integrated and automated system, the MiSeq enables conversion from samples to analyzed data in less than 8 hours. Capable of generating up to 7.0Gb per run, this is the only Next Generation Sequencer that integrates amplification, sequencing and data analysis from a single instrument.

Next Gen Sequencing Services

Whole Genome Resequencing

  • Re-Sequencing genomes where a reference genome is available
  • Randomly shear genomic DNA to create fragment/pair end library using NGS platform protocol.
  • Sequence library on the NGS platform
  • Align against a reference genome and predict variations
  • Annotate variants and identity variant type
  • Deeper analysis of functional and clinical relevant variants

    • Whole Transcriptome Analysis

    • Sequencing based transcriptional profiling
    • Isolate RNA, reverse transcribe generate cDNA libraries with appropriate adapters to create libraries
    • Gene, transcript and splice junction expression
    • Differential expression analysis
    • Novel gene, transcript and splice-junction discovery
    • SNPs Identification and discovery of novel SNPs
    • Promoter, Pathway and Gene-Ontology Analysis
    • Visualization of expression profile and gene-transcript on genome browser

    Exome sequencing

    • Targeted capture of regions of interest from genomes using standard or custom capture probes
    • Generate libraries appropriate for NGS platform
    • Sequence library using the NGS platform
    • Analyze data to identify variations (mutations)
    • Annotate mutation and identify mutation type
    • Comparison of identified mutation with existing common SNP and disease database
    • Visualization of exome data on genome-browser

    Chip Sequencing

    • Capture DNA of interest using appropriate antibody, prepare libraries suitable for next gen sequencing.
    • Alignment of sequence to the reference genome and quality check
    • Peak calling and enrichment analysis
    • Annotation of peaks and identification of target binding sites and consensus motif
    • Pathway and GeneOntology analysis of target genes and promoters
    • Visualization of aligned reads, peaks and target binding sites

    Metagenomics

    • Next-Generation sequencing of bacteria genome
    • Assembly of bacteria genome
    • Protein-coding and non-coding gene prediction
    • Functional Classification of predicted proteins and identification of relevant pathways
    • Comparison with exiting bacteria and other species

    Small RNA Sequencing

    • MicroRNA (miRNA), Piwi-RNA (piRNAs) and other short interfering RNA (siRNA) discovery.
    • Isolate small RNA sample of interest and prepare DNA libraries suitable for NGS
    • Sequence libraries using NGS platforms
    • Perform analysis to identify small RNAs and estimate expression
    • Identify gene targets of expressed small RNAs (especially miRNAs and siRNA)
    • Discovery of novel small RNAs

    Sanger Sequencing Services

    Sanger DNA sequencing services are performed by our professionally trained staff and analyzed with advanced bioinformatics tools. We provide Custom sequencing services for Plasmid DNA, PCR samples, BACs, Primer walking and genotyping. In these areas we provide packages that are tailored to our customer’s specific needs: Quick Single pass DNA Sequencing, Full Sequence Analysis, High Throughput DNA Sequencing and Specialty DNA Sequencing,

    In these areas we provide the following:

    • High quality sequencing read-length >550bp
    • BigDye cleanup for ready to load samples at very affordable rates
    • No additional charge for universal primers
    • Facility to download results from our server
    • Free sample and primer storage for a period of 15 days

    Microbial Identification

    • Bacterial identification using 16S rRNA gene
    • Fungal identification using ITS1-ITS2/28S region
    • Algal identification using 23S